Center Innovation Fund: ARC CIF
Sample Preparation and Processing for Nanopore Life Detection Instruments
Project Description
The technical approach includes physically disrupting samples as a first step in sample preparation. Sample will then delivered for separation based on molecular charge via ion-exchange chromatography. Polyanionic and polycationic polymers, along with other charged molecules, will be eluted off the ion-exchange column for continued downstream processing. The charged particles will then be sorted according to their size via gel-filtration chromatography and delivered to solid-state nanopore device.
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Anticipated Benefits
There is a need for a microfluidic front-end, sample-processing device to enable autonomous solid-state nanopore life detection instruments because current flight instruments can not appropriately preprocess samples prior to analysis. We will incorporate into one single automated microfluidic system: cell lysis, extraction of charged particles and sorting based on size distribution.
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Primary U.S. Work Locations and Key Partners
| Organizations Performing Work | Role | Type | Location |
|---|---|---|---|
Ames Research Center
(ARC)
|
Lead Organization | NASA Center | Moffett Field, California |
| SETI Institute (SETI) | Supporting Organization | Non-Profit Institution | Mountain View, California |
| University of California-Santa Cruz | Supporting Organization |
Academia
Hispanic Serving Institutions (HSI)
|
Santa Cruz, California |
Primary U.S. Work Locations
-
California
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