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Center Innovation Fund: ARC CIF

Adapting RNAseq sample preparation for ISS

Completed Technology Project

Project Introduction

Adapting RNAseq sample preparation for ISS
The primary innovation for this CIF will be the ability to accomplish library preparation of isolated RNA that will enable transcriptional (RNA instead of DNA) sequencing on ISS. Along with optimization of the existing RNA extraction protocols, this will enable on-station whole transciptomic analysis of an organism via RNAseq library prep. The transcriptional sequencing steps can be summarized as follows: 1) RNA Extraction (isolation); 2) RNA conversion to cDNA and amplification of the cDNA (Pre-Amp); 3) Sequencing library preparation (providing genetic molecules of a maximum length of 5,000-8,000 base pairs (bp) and attaching ligation enzymes to the ends of the strands); 4) Sequencing run on the MinION More »

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