Swab-to-Sequence: Real-time Data Analysis Platform for the Biomolecule Sequencer
Project Description
DNA was successfully sequenced on the ISS in 2016, but the DNA sequenced was prepared on the ground. With FY'16 IRAD funds, the same team developed a swab-to-sequencer process for the generation of sequence-ready DNA from environmental samples and demonstrated this process during the NEEMO 21 mission. Additionally, the team has already transitioned key aspects of this process to the ISS as part of the Genes in Space-3 (GiS3) payload. GiS3 has already confirmed the ability to prepare DNA for sequencing in the microgravity environment of space. While these methods continue to be tested on the ISS, a key aspect of this process yet to be developed is a software package to allow for in-flight data analysis. We will leverage software developed for a DOD-funded biosurveillance program and create a customized graphical-user-interface (GUI), offering point-and-click loading of data, parameter adjusting, and launching of analysis with simplistic visualization of results to allow for real-time microbial assessments of the environment.
More »Anticipated Benefits
This technology will benefit a wide range of users. The entire sample-to-answer process will be able to be completed in-flight (or in any environment), and this technology will be available to a wide range of potential users. NASA will specifically benefit, as microbial contamination data will be available in real time and therefore actionable. It is anticipated that other government agencies will adopt this process to support Nanopore sequencing workflows, as NASA is already sharing sample preparation methods with them (for example the CDC).
More »Primary U.S. Work Locations and Key Partners
| Organizations Performing Work | Role | Type | Location |
|---|---|---|---|
Johnson Space Center
(JSC)
|
Lead Organization | NASA Center | Houston, Texas |
| MRIGlobal | Supporting Organization | Industry |
Primary U.S. Work Locations
-
Texas
