In the proposed format, the Hydrosol Concentrator for microgravity (HC-ug) will have direct application to the microbial water monitoring needs of the International Space Station & all crewed national and international space agencies and missions. Further, because small sample sizes are a requirement of rapid microbial detection systems, & because required microbial detection limits for drinking water are extremely low, this need is not anticipated to decrease in the near future. The WetLab-2 initiative will directly benefit from the Hc-ug program, & they will be able to spin off additional tools using this technology. In addition to the needs of the space agency community, many components of the technology developed in the proposed project will also have application to USG earth-based microbial water monitoring applications. The small, zero-power format of the HC-ug system will lend itself to development of fieldable concentration devices for DoD water monitoring needs in austere environments. It will be applicable to field sampling and analysis during outbreak investigations in remote locations, or when sending samples to a laboratory is not acceptable. Water monitoring in developing countries is an important need that could benefit greatly from low-cost, fieldable kits that allow for delivery of a concentrated sample to rapid detection kits. InnovaPrep is already working to identify aligned opportunities within US DoD through DTRA's SOCOM handheld sample prep prgmThe proposed Phase II project will perfect elution fluid formulation and development of our fluid canisters, and improve manufacturing techniques for elution canisters and handheld devices for use in an InnovaPrep biological concentrator for use in a microgravity environment. We expect that the improved performance of our space systems will provide commercial potential for InnovaPrep. The feasibility, usefulness, and market need for commercial InnovaPrep systems that use canned elution fluids and/or hollow fiber concentration has already been demonstrated. As of December 2015, over 150 instruments have been sold or leased worldwide that use our canned fluids, and nearly 10,000 fluid canisters have been manufactured here and sold to those customers. Over 50,000 concentration cells have been sold. This initial success includes early adoption by DoD in the Joint Biological Tactical Detection System (JBTDS), which is now in the early manufacturing and design (EMD) phase. When completely deployed by 2019, over 4500 of those systems will be in daily operation, using up to 4.5 million canisters per year. InnovaPrep has also made significant early progress in commercial markets, and customers include global market leading companies in biopharmaceuticals, commercial products manufacturing, animal health, food & beverage, and other applications of industrial microbiology and life sciences R&D. The sales to these customers are expected to exceed the sales to DoD.