Physical Sciences Inc. (PSI) proposes to develop a novel ophthalmic imaging platform for the characterization and monitoring of visual impairment observed in long-duration space flights. This platform will combine non-invasive measurement of retina/choroid structure and ocular blood flow based on Optical Coherence Tomography (OCT) and wide-field semi-quantitative global flow visualization using Line-scanning Doppler Flowmetry (LSDF). During Phase II a system will be fabricated utilizing the most deeply penetrating waveband around 1060 nm which is especially critical for choroidal imaging. Therefore, the PSI's instrument will address the need for accurate 3D measurement of posterior segment layer thicknesses and volumes, and vascular (retinal and choroidal) topology and flow quantification. This novel imaging platform will enable Phase II imaging studies in animals and human subjects in normal and fluid-shift models of micro-gravity conditions, which are in line with the International Space Station (ISS) mission. Prior PSI experience in developing advanced ophthalmic imaging systems and space-qualified hardware will be leveraged to ensure the successful outcome of this important R&D program.