DNA Extraction and Preparation for a Sequencing Process from Low Biomass Samples (DEAP Seq PLS)
Project Introduction
As we set our sights toward Mars, we must develop the means to perform environmental and medical monitoring in the spaceflight environment. Determining the sequence of DNA is a powerful molecular biology technique that enables medical diagnostics and the identification of microorganisms. A DNA sequencer is being tested on the ISS in 2016 by the proposal team (Biomolecule Sequencer Payload). Sequence-ready DNA, prepared on the ground, will be launched for this testing. The ability to perform inflight sample collection and DNA manipulations, required to generate sequence-ready DNA, from low biomass samples (few cells and/or free DNA, as is the case on the ISS and in planetary protection scenarios) is far from reality. Having the capability to preform inflight sequencing preparation could expand the capabilities of the sequencer to include microbial monitoring, monitoring changes in microbes and humans in response to spaceflight, addressing planetary protection concerns, and detecting DNA-based life elsewhere in the universe.
This project will develop a common process including: 1) optimal swab selection, 2) DNA extraction, 3) DNA manipulation, and 4) the preparation of sequence-ready DNA that is compatible with the sequencer being tested inflight, for cell and free DNA collection from high-fidelity, low biomass samples. The development of a sample collection and preparation process is essential for the sequencer to be used in a capacity beyond testing Earth-prepared DNA samples.
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Anticipated Benefits
The Biomolecule Sequencer is on the Environmental Health Systems Strategic Plan, the ECLSS Environmental Monitoring Gap Closing Roadmap, and is in the planning for proposed robotic missions. However, it cannot be used in any of these programs until a means of purifying and preparing DNA is reality. In addition, this capability is being tracked by a technology watch effort within the Human Research Program. The project team has also made significant strides with the ISS Science Office, NanoRacks, and forward contamination initiatives to further the need for sequencing preparation. The proposal team is experienced in certifying payloads for flight through the Class 1-E process and, once developed, intends to seek avenues for flying this preparation process.
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Primary U.S. Work Locations and Key Partners
| Organizations Performing Work | Role | Type | Location |
|---|---|---|---|
Johnson Space Center
(JSC)
|
Lead Organization | NASA Center | Houston, TX |
Ames Research Center
(ARC)
|
Supporting Organization | NASA Center | Moffett Field, CA |
|
Goddard Space Flight Center
(GSFC)
|
Supporting Organization | NASA Center | Greenbelt, MD |
| MO BIO Laboratories | Supporting Organization | Industry | |
| Oxford Nanopore Technologies | Supporting Organization | Industry |
Primary U.S. Work Locations
-
California
-
Ohio
-
Texas
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Organizational Responsibility
Responsible Mission Directorate:
- Space Technology Mission Directorate (STMD)
Lead Center / Facility:
- Johnson Space Center (JSC)
Responsible Program:
- Center Innovation Fund: JSC CIF
Project Management
Program Director:
Project Manager:
Principal Investigator:
Co-Investigators:
Project Duration
Technology Maturity (TRL)
| Start: | 4 |
| Current: | 4 |
| Estimated End: | 6 |
Technology Areas
Primary:
- TA 6 Human Health, Life Support, and Habitation Systems
- TA 6.1 Environmental Control and Life Support Systems and Habitation Systems
Other / Cross-cutting:
- TA 6 Human Health, Life Support, and Habitation Systems
- TA 6.3 Human Health and Performance
- TA 6.3.1 Medical Diagnosis and Prognosis
