HJ Science & Technology (HJS&T) and Lawrence Berkeley National Laboratory (LBNL) propose a highly integrated, programmable, and miniaturized microfluidic automation platform capable of running rapid and complex synthetic biology and bioengineering processes for engineering life supporting microbes in space exploration missions. Our approach combines the microfluidic automation technology of HJS&T with the novel synthetic biology technologies of 1) combinatorial gene library generation, 2) host transformation, and 3) gene product screening at LBNL and the Joint BioEnergy Institute (JBEI). In Phase I, we have established the feasibility of the proposed microfluidic automation technology by engineering and screening cyanobacterial cells for enhanced production of free fatty acids. In Phase II, we will expand the Phase I microfluidic automation capability to enable automated, metabolic engineering and screening of microbes for enhanced production of other classes of important compounds for in situ resource utilization in NASA space exploration missions: propellant fuels, biopolymers, and pharmaceuticals. We will also build and deliver a Phase II prototype. The successful development of the microfluidic automation technology with its automated and miniaturized platform will lay the groundwork for life supporting waste management and in situ resource utilization capabilities in future NASA manned space exploration missions.