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Center Innovation Fund: ARC CIF

A Robust, Cell-free Production System for On-Demand Protein Synthesis in Space

Completed Technology Project

Project Introduction

A Robust, Cell-free Production System for On-Demand Protein Synthesis in Space
We will develop a new cell-free expression system that functions after rehydrating from a freeze-dried condition. Freeze-dried powder that can be stored or transported at room temperature with reduced volume and mass, thus decreasing mission risk and increasing flexibility in production. Recently D. radiodurans ultrafiltrate (extraction of low molecular size compounds < 3.5 kDa) has shown the capability of protecting freeze-dried enzymes from ionizing radiation suggesting that D. radiodurans cell-free lysate is most likely contains natural anti-oxidants[1]. No work has been reported on developing a cell-free expression system with extremophiles. We will to develop an extremely stable cell-free expression system using D. radiodurans cell lysate and evaluate the performance of protein expression after freeze-drying and rehydration More »

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