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Center Innovation Fund: ARC CIF

Methane Metabolism by Yeast

Completed Technology Project

Project Introduction

Methane Metabolism by Yeast
Our Technical Approach is to move the soluble Methane Monooxygenase system (sMMO) from methanotrophic bacteria into Pichi). sMMO consists of a hydroxylase (MMOH) assembled from 3 polypeptides, a reductase (MMOR), and a regulatory protein (MMOB). Thus 5 genes must be introduced into Pichia to enable oxidation of methane to methanol. Methanol then enters the natural metabolism of Pichia. Methods for introducing genes into Pichia are well developed, and we will apply these techniques to introduce the genes of the sMMO system into Pichia. We will judge the success of our efforts by growing Pichia containing sMMO with methane as a sole carbon source and comparing this to control strains. We will also measure incorporation of 13C methane into biomass. More »

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